ABSTRACT
AIM: The aim of this study was to determine the association between IL-1B (+3954) gene polymorphism and chronic periodontitis in a sample of the south Indian population. SETTINGS AND DESIGN: This study employed a cross-sectional design involving individuals from the state of Tamil Nadu in the southern part of India. MATERIALS AND METHODS: Genomic DNA was obtained from the white blood cells of 30 patients with chronic periodontitis (18 males and 12 females) and 31 healthy controls (20 males and 11 females). The age of the subjects ranged from 30 to 55 years old and all were non smokers. DNA was amplified using the polymerase chain reaction (PCR) with specific primers flanking the locus +3954 of IL-1beta gene and analyzed by 3% agarose gel electrophoresis. STATISTICAL ANALYSIS: A Chi-square test was used to determine the genotype distribution between the groups and the relative risk was estimated with a 95% confidence interval. RESULTS AND CONCLUSION: The chronic periodontitis group displayed a higher percentage of T allele, even though it was not statistically significant. The relative risk analysis between genotypes showed that the risk was higher for the CT genotype compared with the CC genotype and the risk was significant. In conclusion, our data suggested that there was no significant association between IL-1beta (+3954) gene polymorphism and chronic periodontitis in the south Indian population.